固定化GL-7-ACA酰化酶,Immobilized GL-7-ACA Acylase
1)Immobilized GL-7-ACA Acylase固定化GL-7-ACA酰化酶
1.Preparing D-7-ACA with tri-enzyme method(Immobilized D-amino acid oxidase,Immobilized GL-7-ACA Acylase,Immobilized deacetyl esterase) is an original,sustainable and brand new technology in promoting the development of green pharmaceutical industry in our country.三酶(D-氨基酸氧化酶、固定化GL-7-ACA酰化酶和固定化去乙酰基酯酶)法制备D-7-ACA工艺,属国内首创的新技术、新工艺,有利于我国绿色制药工业的发展,是一种可持续的工艺路线。
2)GL-7-ACA acylaseGL-7-ACA酰化酶
1.7β (4-Carboxybutanamido) cephalosporanic acid acylase (GL-7-ACA acylase) was purified from cell-free extracts of a recombinant, CU334 by a procedure invoiving ammonium suifate fractionation and successive column chromatography on DEAE-Sephadex A-50, DEAE-cellulose DE-52, Sephadex G-200 and Hydroxylapatide with 22-fold purification, 4.CU334是高表达GL-7-ACA酰化酶工程菌,其菌悬液用超声波处理后,经硫酸铵分级沉淀、DEAE-Sephadex A-50离子交换柱层析、DEAE—纤维素DE-52柱层析、Sephadex G-200凝胶过滤及羟基磷灰石吸附柱层析等步骤,得到了凝胶电泳均一的GL-7-ACA酰化酶蛋白,纯化了22倍,得率4。
2.This paper has determined the K_m and V_(max) of native immobilized GL-7-ACA acylase and free enzymes.通过对国产固定化GL-7-ACA酰化酶和游离酶的表观米氏常数(Km)及最大反应速度(Vm)的测定。
3.By taking advantage of uniform design and using national materials, the formula of fermentation media of GL-7-ACA acylase was optimized and gained better effect.采用国产原料,应用均匀设计优选试验方法,对GL-7-ACA酰化酶生产用的发酵培养基配方进行了优化,取得了良好的效果,最终摇瓶效价达3919。
英文短句/例句

1.Study on Fermentation Technology of GL-7-ACA Acylase Production by Genetically Engineered Bacterium and Enzyme Engineering;GL-7-ACA酰化酶基因工程菌的发酵工艺优化及酶工程研究
2.Study on Producing a New Pharmaceutical Intermediate D-7-ACA with Enzymatic Technology酶法制备新型医药中间体D-7-ACA工艺研究
3.Research on techniques of β-glucosidase immobilized in ACA microcapsuleβ-葡萄糖苷酶ACA微胶囊固定化实验研究
4.Optimization of Conditions in Decolorization of Direct Fast Turquoise Blue GL Catalyzed by Laccase from Ganoderma lucidum灵芝漆酶催化直接耐晒翠蓝GL脱色条件的优化
5.Application and Market of Pharmaceutical Intermediates of 7-ADCA and 7-ACA;7-ADCA和7-ACA的应用与市场
6.Study on the Interaction of Lysozyme and Chrysin-7-phosphated Amino Acid Esters by ESI Mass SpectrometryESI质谱法对黄酮-7-磷酰化氨基酸酯与溶菌酶相互作用的研究
7.Purification and Characterization of a Novel Autoproteolytic Antithrombin,AA-ACA-I,from the Snake Venom of Agkistrodon acutus蕲蛇蛇毒中一种新型自降解抗凝血酶AA-ACA-I的纯化和表征(英文)
8.A novel intermediate of Cephalosporins D-7-ACA头孢类抗生素的新型中间体D-7-ACA
9.Enzymatic Synthesis of 1-O-glycyl-3-O-tetradecanylglycerol酶催化1-O-甘氨酰-3-O-十四烷酰化甘油的合成
10.Research on the Effect and Mechanism of ACA on Human Breast Carcinoma Cell Line MCF-7ACA对人乳腺癌细胞MCF-7作用及其机制的实验研究
11.Immobilization of N-carbamyl-D-amino acid amidohydrolaseN-氨甲酰基-D-氨基酸酰胺水解酶的固定化工艺
12.The Acetylation of Thymosin α1 is Independent on RimL in Escherichia coli胸腺素α1的乙酰化修饰不依赖于乙酰转移酶RimL
13.Microbial Transformation of 7-epi-10-DeacetylbaccatinⅢto 10-DeacetylbaccatinⅢ(10-DABⅢ)7-表-10-去乙酰巴卡亭Ⅲ的微生物转化(摘要)
14.Acylases-catalyzed Michael Addition of N-heterocycle Derivatives;酰化酶催化C-N键Michael加成研究
15.Immobilization of Penicillin G Acylase--Using Layer Material;层状材料固定化青霉素酰化酶的研究
16.etyl-CoA transacetylase乙酰辅酶A转乙酰酶
17.Extraction of 10-Deacetylpaclitaxel,the Enzymic Hydrolysis Products of 7-Xylosyl-10-Deacetylpaclitaxel,by Macroporous Adsorption Resin大孔吸附树脂提取7-木糖-10-去乙酰紫杉醇酶解产物10-去乙酰紫杉醇
18.Studies on Immobilized L-asparaginase Based on Polyacrylamide/N,N'-methylenebisacrylamide;聚丙烯酰胺/N,N'-亚甲基双丙烯酰胺固定化L-天门冬酰胺酶的研究
相关短句/例句

GL-7-ACA acylaseGL-7-ACA酰化酶
1.7β (4-Carboxybutanamido) cephalosporanic acid acylase (GL-7-ACA acylase) was purified from cell-free extracts of a recombinant, CU334 by a procedure invoiving ammonium suifate fractionation and successive column chromatography on DEAE-Sephadex A-50, DEAE-cellulose DE-52, Sephadex G-200 and Hydroxylapatide with 22-fold purification, 4.CU334是高表达GL-7-ACA酰化酶工程菌,其菌悬液用超声波处理后,经硫酸铵分级沉淀、DEAE-Sephadex A-50离子交换柱层析、DEAE—纤维素DE-52柱层析、Sephadex G-200凝胶过滤及羟基磷灰石吸附柱层析等步骤,得到了凝胶电泳均一的GL-7-ACA酰化酶蛋白,纯化了22倍,得率4。
2.This paper has determined the K_m and V_(max) of native immobilized GL-7-ACA acylase and free enzymes.通过对国产固定化GL-7-ACA酰化酶和游离酶的表观米氏常数(Km)及最大反应速度(Vm)的测定。
3.By taking advantage of uniform design and using national materials, the formula of fermentation media of GL-7-ACA acylase was optimized and gained better effect.采用国产原料,应用均匀设计优选试验方法,对GL-7-ACA酰化酶生产用的发酵培养基配方进行了优化,取得了良好的效果,最终摇瓶效价达3919。
3)immobilized glutaryl-7-ACA acylase (GA)固定化戊二酰-7-ACA基酰化酶(GA)
4)Immobilized GL-7ACA-acylase固定化GL-7ACA酰化酶
5)GL-7ACA acylaseGL-7ACA酰化酶
1.Glutaryl-7-aminocephalosporanic acid acylase(GL-7ACA acylase) is one of important industrial enzymes for the production of 7-aminocephalosporanic acid(7-ACA),in this paper,a new metal chelating carrier(EIPARG-IDA-Co2+)was prepared to immobilize GL-7ACA acylase.采用金属鳌合亲和层析技术,以EIP-ARG-IDA-Co2+为载体,从可溶性总蛋白中一步分离纯化具有His-tag标签的GL-7ACA酰化酶。
6)ACA microcapsule immobilizationACA微胶囊固定化
延伸阅读

固定①不变动或不移动的(跟‘流动’相对):~职业丨~资产。②使固定:把学习制度~下来。