核酸探针,nucleic acid probe
1)nucleic acid probe核酸探针
1.Progress on Molecular Biology and Nucleic Acid Probe of Porcine Parvovirus;猪细小病毒分子生物学与核酸探针的研究进展
2.Cloning, Sequence Analysis and Studies of Nucleic Acid Probe of the TK Gene of Infectious Laryngotracheitis Virus (ILTV) Inner Mongolia Strain;传染性喉气管炎病毒内蒙98a株TK基因的克隆、序列分析及核酸探针的研究
3.Nucleic acid probe technique and its application are reviewed with 56 references.本文对核酸探针技术进行了较全面的综述,介绍了核酸探针的制备及非放射性标记方法。
英文短句/例句

1.Study on the Aggregation of Lable-free Nucleic Acid Probe Induced by DNA核酸介导的新型免标记核酸探针的集聚
2.Detection of Yersinia pestis with Peptide Nucleic Acid Probes应用肽核酸探针检测鼠疫耶尔森氏菌
3.Designing and Using the Penicillium Marniffei Nucleate Probe;马尔尼菲青霉核酸探针的设计与应用初探
4.Monitoring Nucleic Acids Replication and Polymerase Activity and Detecting Point Mutation Based on Nucleic Acid Probes;核酸探针用于核酸复制与酶活性的实时监测及点突变分析
5.Detection of Nucleic Acid of Poultry Virus with Nucleic Acid Probe and Molecule Dot Blot Hybridization Technique;用核酸探针和分子斑点杂交技术检测家禽病毒的核酸
6.Detection of HAV Vaccine Titre with the Nucleic Acid Probe应用核酸探针检测甲型肝炎疫苗的滴度
7.Kinetics Model of Nucleic Acid Hybridization Studied by QCM石英晶体微天平研究核酸探针的杂交反应模型
8.Study on Novel Naphthalimide Nucleic Probes and Their Photochemical Reactions;萘亚胺新型核酸探针及其光化学反应的研究
9.Study on the Nucleic Acid Probe Analysis by Chemiluminescence and Resonance Light Scattering Technique;核酸探针的化学发光及共振光散射分析
10.Studies on the Identification of Recombinant Virus vFV282 with Nucleic Acid Probe and PCR Methods;核酸探针与PCR方法对重组病毒vFV282株的鉴定研究
11.Research of Constructing the New Genechip of Nucleotide Probe Extension in Situ;构建新型核酸探针原位延伸基因芯片的研究
12.The Study of DNA Probe Detection of the Channel Catfish Virus斑点叉尾鮰疱疹病毒核酸探针检测方法的研究
13.Establishment of Nucleic Acid Probe Method to Detect Escherichia coli O157:H7大肠杆菌O157:H7核酸探针检测方法的建立
14.Preparation and Application of M Gene Nucleic Acid Probe of Swine Influenza Virus猪流感病毒M基因核酸探针的制备与应用
15.Expression of B and C Antigenic Sites of TGEV Spike Gene in Prokaryocyte and Preparation of Nucleic Acid Probe at 5 Terminus;TGEV S基因B和C抗原位点片段的原核表达及5’核酸探针制备
16.Design of Nucleic Acids Probes and Its Significant Application in Disease Detection and Enzyme Analysis;核酸探针的设计及其在疾病检测和酶学研究中的应用
17.Study on the Detection of Thrombin Based on Aptamers;基于核酸适体探针检测凝血酶的研究
18.Determination of DNA Using Brilliant Crystal Blue as Electrochemical Probe灿烂甲酚蓝电化学探针测定脱氧核糖核酸
相关短句/例句

DNA probe核酸探针
1.The detection of medical fungi using DNA probe labelled with long arm photobiotin;应用长臂光敏生物素核酸探针检测医学真菌的研究
2.Partial cloning of the genome library of white spot syndrome virus from Penaeus monodon and DNA probe detection methods;斑节对虾白斑综合症病毒部分基因组文库及核酸探针检测法
3.The purified DNA fragment was labelled by biotin as a DNA probe.以pUC19质粒载体将此片段克隆,用EcoRI和HindⅢ酶切此重组质粒,回收克隆片段后,制成生物素标记的核酸探针
3)nucleic acid probes核酸探针
1.We reported here a new method for detection of radioactivity of radiolabeled nucleic acid probes.4 mol/L的NaOH为层析液,进行膜上层析,分离经放射性同位素标记的核酸探针和未掺入的放射性游离单核苷酸,再经放射性强度测定即可计算出标记后的核酸探针的放射性比活。
4)nucleotide probe核酸探针
1.Development and primary application of nucleotide probe to detect canine coronavirus;核酸探针检测犬冠状病毒方法的建立和初步应用
2.Detection of nucleotide probe of type 1 pili pilA gene of avian Escherichia coli labeled with digitoxin hybridizated to avian Escherichia coli isolated strain;地高辛标记大肠杆菌1型菌毛结构基因核酸探针对鸡大肠杆菌分离株的检测
3.Specificity tests were carried out with the nucleotide probe to detect the reverse transcription(RT) products of CDV,NDV,MV and CEF cell without .以RT-PCR技术从CDV基因中扩增出了一段长度为430 bp的核苷酸cDNA,并制备出地高辛标记的CDV核酸探针
5)Peptide nucleic acid probe肽核酸探针
6)Olignucleotide prob核苷酸探针
延伸阅读

生物素标记核酸探针技术分子式:CAS号:性质:这是一种非放射性标记技术。主要利用了生物素(biotin)和亲和素(avidin)这两种分子的独特性质。亲和素是一种碱性的四聚体糖蛋白,其分子量为68000,pI10.5。每个亚基都能结合一个生物素分子。生物素与亲和素的结合只需要生物素的脲基环部分。因此可将其戊酸侧链通过酰胺键与核酸分子相连,构成生物素标记的核酸探针、标记探针与固定在滤膜(硝酸纤维素滤膜或尼龙膜)上或固定在组织细胞或染色体原位的核酸分子的同源序列互补杂交。探针和待侧核酸在杂交前若为双链,应先变性成单链,然后用偶联有酶或荧光物质的亲和素的探针与待测核酸的片段互补杂杂,使杂交部位显色(加入相应的酶底物)或产生荧光。灵敏度可达1~10pg水平,与放射性探针法相近。如果进一步改进,甚至可超过后者。