1)siRNA短干扰RNA
1.The dsRNA introduced or generated in cells is subjected to digestion with a dsRNA-specific endonuclease, Dicer, into 21-25 nucleotide (nt) RNA duplexes, and the resultant duplexes, referred to as short interfering RNAs (siRNAs) duplexes, function as essential sequence specific mediators of RNAi in the RNA-induced silencing complexes (RISC).DsRNA进入细胞后被特异性dsRNA核酸酶(Dicer)切割成多个21-25bp的短干扰RNA(short interfering RNAs，siRNAs)，siRNAs与一些核酸酶结合形成RNA诱导的沉默复合体(RNA-induced silencing complex，RISC)，由RISC介导同源mRNA的降解，属于转录后基因沉默(post-transcriptional gene silencing，PTGS)的一种。
2)Small interfering RNA短干扰RNA
1.The activated T cells were transfected with chemical synthesized small interfering RNA(siRNA)sequences for RORγt gene using liposomes.方法:健康成人外周血单个核细胞用CD3单克隆抗体和IL-2激活和扩增T细胞,活化T细胞用脂质体法转染人工合成的RORγt基因特异性短干扰RNA(siRNA)序列3个片段(RORγt-siRNA-982,-1026,-1197),RT-PCR半定量法检测活化T细胞转染siRNA后RORγt基因表达,流式细胞仪检测不同T细胞亚群产生IFN-γ细胞的比例。
3)short interfering RNA短干扰RNA
1.Objective To develop a linear DNA vector approach targeting the Ku80 gene sequence and investigate the inhibitory effect of short interfering RNA(siRNA) on Ku80 expression.目的针对内源性Ku80基因序列体外构建短干扰RNA(siRNA)线性DNA转录载体,观察其在细胞内产生的siRNA对该基因表达的影响。
4)Short hairpin RNA短发夹状干扰RNA
1.Construction of specific short hairpin RNA (shRNA) for Nucleostemin by T7 RNA polymerase promoter in vitro;T7启动子介导体外构建Nucleostem in特异性短发夹状干扰RNA
2.HL-60 cells were taken as the model,and were directly transfected with nucleostemin short hairpin RNA(NS-shRNA).用T7启动子介导体外合成两条短发夹状干扰RNA(NS-shRNA),以其中沉默NS基因作用强的一条NS-shRNA转染HL-60细胞,同时以与NS基因序列无关小干扰RNA转染HL-60细胞作对照,应用RT-PCR检测被转染的HL-60细胞NS-mRNA抑制效果,倒置显微镜下观察活体细胞形态变化,Wright-Giemsa染色观察细胞胞体和细胞核形态学变化,以流式细胞仪(FCM)和TdT介导的dUTP缺口末端标记技术(TUNEL)测定凋亡细胞数并计算凋亡阳性率。
英文短句/例句
1.The Effect of Short Hairpin RNA of Nuclear Factor Kappa B on the Apoptosis of Hepatic Stellate Cell;核因子-κB亚单位p65短发夹状干扰RNA对肝星状细胞凋亡的影响
2.The Research about Interference of Nucleostemin-Specific Short Hairpin RNA in Vitro and in Vivo;核干细胞因子特异性短发夹状RNA的体内外干扰作用研究
3.Inhibition of Bcl-2 by shRNA to Induce Apoptosis in Human Lens Epithelial Cells短发夹状RNA干扰Bcl-2诱导晶状体上皮细胞凋亡的实验研究
4.Short Hairpin RNA Silences HMGB1 Gene and Affets Proliferation in Endometrial Cancer Cell Line短发夹状RNA干扰对子宫内膜癌细胞中HMGB1基因表达及细胞增殖的影响
5.RNA interfering effects of CCL20 shRNA expressing vectors on human embryonic kidney cellsCCL20基因短发夹RNA表达载体转染人胚胎肾细胞的干扰效应
6.Construction and identification of human Nuclear factor kappa B P65 siRNA retrovirus expression vector in vitro针对核因子-κBP65基因的短发夹干扰RNA逆转录病毒载体构建与鉴定
7.RNA interference targeting hypoxia inducible factor-1α in BGC-823 cells inhibits cell proliferation and induces cell apoptosis短发夹RNA干扰胃癌低氧诱导因子-1α表达及其临床意义
8.Interference effects of short-hairpin RNA on focal adhesion kinase on the sensitivity to 5-fluorouracil of multicellular spheroids of colonic carcinoma短发夹RNA干扰黏着斑激酶对结肠癌多细胞球氟尿嘧啶敏感性的影响
9.Effect of RNA Interference Targeting of PI3K on the Proliferation of Vascular Smooth Muscle Cell and the Intimal Hyperplasia;靶向PI3K短发卡RNA干扰防治血管移植术后再狭窄
10.Mechanism of Short Hairpin RNA Silence NF-κB Promotion Apoptosis of Hepatic Stellate Cell;应用短发夹状RNA沉默NF-κB基因促进肝星状细胞凋亡机制研究
11.Construction and Identification of Plasmid Mediated RNA Interference Targeting Egr-1针对小鼠早期生长反应因子-1发夹结构小干扰RNA载体的构建及其干扰效率鉴定
12.Effect of Suppressing COX-2 Expression by shRNA on Adhesion and Invasion of Human Hepatocellular Carcinoma Cell;短发夹状RNA抑制COX-2表达对人肝癌细胞株黏附侵袭力的影响
13.Construction and Identification of Multidrug Resistance Gene MDR1 Short Hairpin Expression Plasmids;多药耐药基因MDR1短发夹状RNA表达质粒的构建与鉴定
14.Reversal of Leukemia Multidrug Resistance by Sequence-Specific Short Hairpin RNA序列特异性短发夹状RNA逆转白血病多药耐药性研究
15.Biosynthesis and function verification of short hairpin RNA targeting to Influenza A virus抗甲型流感病毒短发夹状RNA分子的体外生物合成及功能鉴定
16.Short hairpin RNA inhibits HPV16 E6 oncogene expression in cervical cancer Siha cells短发夹状RNA抑制子宫颈癌Siha细胞HPV16 E6基因的表达
17.Construction of the shRNA expression vectors targeting survivinSurvivin靶向的短发夹RNA表达载体的构建
18.Studies of RNA Interference on SARS-associated Coronavirus Spike Glycoprotein;SARS冠状病毒刺突糖蛋白的RNA干扰研究
相关短句/例句
Small interfering RNA短干扰RNA
1.The activated T cells were transfected with chemical synthesized small interfering RNA(siRNA)sequences for RORγt gene using liposomes.方法:健康成人外周血单个核细胞用CD3单克隆抗体和IL-2激活和扩增T细胞,活化T细胞用脂质体法转染人工合成的RORγt基因特异性短干扰RNA(siRNA)序列3个片段(RORγt-siRNA-982,-1026,-1197),RT-PCR半定量法检测活化T细胞转染siRNA后RORγt基因表达,流式细胞仪检测不同T细胞亚群产生IFN-γ细胞的比例。
3)short interfering RNA短干扰RNA
1.Objective To develop a linear DNA vector approach targeting the Ku80 gene sequence and investigate the inhibitory effect of short interfering RNA(siRNA) on Ku80 expression.目的针对内源性Ku80基因序列体外构建短干扰RNA(siRNA)线性DNA转录载体,观察其在细胞内产生的siRNA对该基因表达的影响。
4)Short hairpin RNA短发夹状干扰RNA
1.Construction of specific short hairpin RNA (shRNA) for Nucleostemin by T7 RNA polymerase promoter in vitro;T7启动子介导体外构建Nucleostem in特异性短发夹状干扰RNA
2.HL-60 cells were taken as the model,and were directly transfected with nucleostemin short hairpin RNA(NS-shRNA).用T7启动子介导体外合成两条短发夹状干扰RNA(NS-shRNA),以其中沉默NS基因作用强的一条NS-shRNA转染HL-60细胞,同时以与NS基因序列无关小干扰RNA转染HL-60细胞作对照,应用RT-PCR检测被转染的HL-60细胞NS-mRNA抑制效果,倒置显微镜下观察活体细胞形态变化,Wright-Giemsa染色观察细胞胞体和细胞核形态学变化,以流式细胞仪(FCM)和TdT介导的dUTP缺口末端标记技术(TUNEL)测定凋亡细胞数并计算凋亡阳性率。
5)Short/small interference double-strand RNA短小干扰双链RNA
6)short hairpin RNA interference短发夹RNA干扰
延伸阅读
感染性RNA病原RNA分子式：CAS号：性质：又称感染性RNA病原RNA；壳病毒，是一种和病毒(virus)相似的感染性颗粒。为无蛋白外壳的单链RNA，分子量1.1×105～1.3×105。它是比已知病毒都小的能在宿主细胞内自主复制的病原体之一。已知的近20种类病毒中，大部分已测得了一级结构，都是无蛋白外壳的共价闭合的单链环状RNA分子。在天然状态下类病毒RNA以高度碱基配对的棒状结构形式存在。最先是由T. O. Diener等人(1969)在马铃薯纤块茎病(potato spindle tuber disease)的病株上首先发现的，在电镜下可见到这RNA分子呈50nm长的杆状分子，共有359个碱基对，并证实是游离的RNA，为此正式命名为类病毒。它通常在宿主细胞核内，借助汁液传染，分子量75000～130000，比最小病毒还小80倍。后又相继在菊花矮缩病(chrysanthemum stunt)、菊花绿斑病(chrysanthenum chlorotic mottle)、柑橘剥皮病(citrus excortis)等患病植株中分离到低分子量的病原RNA。推测它也可能存在于其他植物、动物甚至人体内。绝大部分类病毒均具有共同的结构特征：(1)位于棒状结构中心有一个高度保守的序列；(2)靠近这一保守中心区的左侧有一个多聚嘌呤区；(3)棒状结构左侧序列保守性强，右侧变异性大。它可能是通过核苷酸序列或结构改变直接与寄主细胞相互作用、干扰细胞的代谢而致病。对类病毒的研究可能为揭示生命起源和进化、生命过程的实现等生命科学的重大理论问题作出贡献。
