1)Chromatin immunoprecipitation染色质免疫沉淀
1.AIM: To construct a new method named PolII-ChIP-PE- MS (RNA polymerase II-Chromatin immunoprecipitation- primer extension-mass spectrum), and to validate the reliability of this method in the study of single nucleotide polymorphism (SNP) allele-specific quantification o RNA polymerase loading.目的:建立RNA多聚酶Ⅱ-染色质免疫沉淀-引物延伸-飞行时间质谱技术(PolⅡ-ChIP-PE-MS),并利用该方法检测SNP等位特异磷酸化PolⅡ结合量,为活体状态下研究疾病关联基因SNP的功能研究提供一种高通量的研究手段。
2.After the cells were cross-linked by formaldehyde and chromatin was sonicated by ultrasonic, we performed RNA polymerase Ⅱ-chromatin immunoprecipitations (PolⅡ-ChIP) to precipitate the promoter sequences and validated them by PCR.方法常规培养和刺激HepG2和L02细胞,甲醛固定细胞,超声剪切染色质,采用利用PolⅡ CTD末端Ser5特异性抗体进行染色质免疫沉淀(PolⅡ-ChIP),针对文献报道的8个可能启动子区设计引物进行PCR扩增,鉴定ESR1基因启动子的使用情况。
3.The binding of Ezh2 and H3K27me3 on the Ngn 1 promoter was analyzed using chromatin immunoprecipitation assay.方法通过Western印迹检测全反式维甲酸(RA)诱导TuJ1蛋白的表达;通过染色质免疫沉淀技术检测Ngn1基因启动子区Ezh2的结合与H3K27三甲基化的变化;通过实时定量RT-PCR检测RA诱导P19细胞分化后Ezh2mRNA表达。
英文短句/例句
1.Identification of the Downstream Targets of Nanog by Chromatin Immunoprecipitation染色质免疫沉淀法筛选转录因子Nanog调控的目的基因
2.Preparation of Polyclonal Antibody Against N-terminal of Human Elp3 and Its Application in Chromatin Immunoprecipitation抗人Elp3的N末端抗体制备及在染色质免疫沉淀中的应用
3.Genome-wide analysis of histone H3 lysine 27 trimethylation by ChIP-chip in gastric cancer采用染色质免疫沉淀联合芯片技术分析胃癌全基因组蛋白H3K27三甲基化水平
4.COMPARISON OF IMMUNOENZYMATIC STAINING TECHNIQUE (IEST), DOUBLE GLUING STRIP CIRCUMOVAL PRECIPITIN TEST(DGS-COPT) AND CONVENTIONAL CIRCUMOVAL PRECIPITIN TEST(CV-COPT) FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA免疫酶染色试验及双面胶纸条法环卵沉淀试验诊断血吸虫病的比较
5.Further Study on Immunoenzy matic Staining Technique(IEST)and Double Gluing Strip Circumoral Precipitin Test(DGS-COPT)for Diagnosis of Schistosomiasis Japonica免疫酶染色试验和双面胶纸条法环卵沉淀试验诊断血吸虫病的进一步研究
6.immunoperoxidase staining免疫过氧化物酶染色
7.Protein-protein Interaction between Membrane and Nucleocapsid Protein of Avian Infectious Bronchitis Virus Studied by Yeast Two-hybrid System and Co-immunopreciptation Method用酵母双杂交和免疫共沉淀研究禽传染性支气管炎病毒N、M蛋白相互作用
8.Research Protein-protein Interaction between S1 Glycoprotein and Membrane Protein of Avian Infectious Bronchitis Virus by Yeast Two-Hybrid System and Co-immunoprecipitation酵母双杂交、免疫共沉淀研究禽传染性支气管炎病毒S1、M蛋白相互作用
9.Amyloid deposits were positive for Congo Red(15/15).淀粉样沉积物刚果红染色阳性(15/15)。
10.It is revealed that in double diffusion and immunoelectrophoresis, the product yielded a single precipitating line.以双扩及免疫电泳鉴定,均呈现单一沉淀线。
11.Image analysis of GFAP expressed astrocytes in the subjects with primary brainstem injury;脑干星形细胞胶质原纤维酸性蛋白免疫组化染色的图像分析
12.Immunohistochemical study of substance P in the throat and lungs in cases died from anaphylactic shock;过敏性休克死亡尸体咽喉及肺组织中P物质免疫组化染色观察
13.Quality control of immunohistochemical stainiginslice of puncture liver tissues免疫组化染色质量控制在肝脏穿刺组织切片中的应用
14.Lake pigment: A pigment made by precipitation of a soluble dye on an inorganic base to render it insoluble in water.色淀颜料:可溶性染料在无机碱中沉淀而生的颜料，它不溶于水。
15.Serial Studies on C4d by Immunohistochemical Staining;补体成分C4d免疫组化染色的系列研究
16.An immunohistochemical study of bFGF and COX2 after human brain contusion.;人脑挫伤后bFGF和COX2免疫组化染色观察
17.The tyrosine phosphorylation of leptin receptor was revealed by immuno precipitation.应用免疫沉淀法检测瘦素受体的酪氨酸磷酸化程度。
18.Developing a Method of Purifying High Active Bluetongue Virus with Agarose Protein A in Reverse Co-immunoprecipitation;琼脂糖Protein A反向免疫共沉淀技术纯化高活性BTV方法的建立
相关短句/例句
chromatin immunoprecipitation assay染色质免疫沉淀技术
1.Objective To establish a Chromatin immunoprecipitation assay to analyze transcriptional regulation of gene expression in hepatocytes.目的建立一种染色质免疫沉淀技术(ChIP)分析肝细胞基因表达的转录调控方法。
2.Now, chromatin immunoprecipitation assay (ChIP) is ideally suited for investigating DNA-protein interactions in vivo.染色质免疫沉淀技术(chromatin immunoprecipitation assay,简称ChIP)是目前唯一研究体内DNA与蛋白质相互作用的方法。
3.Nowadays, in comparison with other methods, chromatin immunoprecipitation assay (ChIP) is ideally suited for studying DNA-protein interactions in vivo.与其他方法相比,染色质免疫沉淀技术(chromatin immunoprecipitation assay,ChIP)是一种近来研究体内DNA与蛋白质相互作用的最好方法之一。
3)chromatin immunoprecipitation assay (ChIP)染色质免疫沉淀技术(ChIP)
4)chromatin immunoprecipitaion (ChIP)染色质免疫沉淀(ChIP)
5)Chromatin immunoprecipitation染色质免疫共沉淀
1.Here we adopted Chromatin immunoprecipitation linked to microarrays(ChIP-chip),for the first time,to profile the variations in H3K9me3 in CpG island regions in squamous carcinomas of the cervix patients and controls\' ChIP-qPCR is used to validate the microarray results.方法:收集宫颈鳞癌组织5例及对照组织5例,采用染色质免疫共沉淀联合芯片技术(ChiP-chip)在全基因组范围内对宫颈鳞癌组织及正常宫颈组织中的组蛋白H3K9me3进行高通量的筛选,获得宫颈鳞癌组织中H3K9me3的表达图谱。
6)chromatin immunoprecipitation(ChIP) assay染色质免疫沉淀实验
延伸阅读
放射免疫沉淀反应放射免疫沉淀反应 是用放射性同位素标记的抗体或抗原进行免疫沉淀反应的一种方法。
