1)hTERT promoterhTERT启动子
1.Changes of biological character of SKOV3 cells transfected with HSV-tk gene under the control of hTERT promoter;hTERT启动子调控的HSV-tk基因逆转录病毒载体的构建及SKOV3转染细胞的生物学特性观察
2.Aim: To investigate the effect of ganciclovir and topotecan on SKOV3 cell transfected HSV tk under the control of hTERT promoter.目的 :探讨更昔洛韦 (GCV)与拓扑替康 (TPT)单独或联合应用对携带有hTERT启动子调控的HSV tk基因的SKOV3细胞生长的影响。
3.METHODS: The hTERT promoter and TK gene were cloned, andwhich was connected to the PGL3 carrier after enzyme digestion, toconstruct the hTERTp/PGL3、TK/PGL3、hTERTp/TK/PGL3 recombinantcarriers, and then to construct the CMV/TK/PGL3 recombinant carrierswith the similar method and regard CMV a.方法：克隆hTERT启动子及TK基因，酶切后连接到pGL3载体上，构建hTERTp／pGL3，TK／pGL3，hTERTp／TK／pGL3重组载体。
英文短句/例句
1.Effect of Expression of p53 Gene Driven by hTERT Gene Promoter on T24 Apoptosis;hTERT启动子调控p53基因表达对T24细胞凋亡的作用
2.Effect of Bax targeting regulated by hTERT promoter on apoptosis of salivary adenoid cystic carcinoma cellshTERT启动子靶向性驱动Bax诱导腺样囊性癌细胞凋亡机制的探讨
3.Killing Effect of HSV-TK Gene Driven by a Human Telomerase Reverse Tran-scriptase Gene Promoter on Human Pancreatic Cancer Cell Line Patu 8988hTERT启动子驱动的HSV-TK基因对人胰腺癌细胞patu8988的杀伤作用
4.The Selective Killing Effect of Adenoviral Mediated CD/5-FC Suicide Gene System Controlled by hTERT Promoter on Ovarian Cancer Cells;hTERT启动子调控腺病毒介导的CD基因治疗卵巢癌的实验研究
5.Killing Efficiency of Human Ovarian Cancer Cells by HSV-tk/ GCV System under the Control of hTERT Promoter Combined with Topotecan;hTERT启动子调控的HSV-tk基因治疗系统对卵巢癌细胞的作用
6.Experimental Study on the Effect of hTERT Promoter Regulated Vecter Targeting TRALL in Cervical Cancer Cells;hTERT启动子调控TRAIL基因表达载体诱导宫颈癌细胞凋亡的研究
7.Study on Adenovirus with hTERT Promoter Interfering Expression of ERCC1 to Reverse Cisplatin Resistance in Ovarian Cancer Cell Lines;带hTERT启动子的腺病毒干扰ERCC1基因表达逆转卵巢癌顺铂耐药的研究
8.Study on the Apoptosis Effect of hTERT Promoter Regulated Tumor Targeting Trail on Breast Cancer CellshTERT启动子调控的TRAIL基因诱导乳腺癌细胞凋亡作用的研究
9.Analysis to DNA Methylation Status of the Promoter of Human Telomerase Reverse Transcriptase (hTERT) in Gastric Carcinogenesis胃癌形成过程中hTERT启动子甲基化状态变化分析研究
10.hTERT Promotor Associated HSV-tk/GCV System Therapy Effect on Nude Mice Xenografts Model of Human Prostate CancerhTERT启动子联合HSV-tk/GCV对人前列癌裸鼠移植瘤的治疗作用
11.A Study on Cloning Core-sequence of hTERT Promoter and Its Targeting Transcriptional Activity in Lung Cancer CellshTERT启动子核心序列的克隆及其在肺癌细胞中靶向转录活性的研究
12.Inhibitory effect of hTERT promoter requlated turmor targeting TRAIL on breast cancerhTERT启动子调控的TRAIL基因对乳腺癌细胞株的杀伤作用
13.TK gene targeted therapy mediated by the human telomerase promoter for transplanted tumor of nasopharyngeal carcinoma in vivo in nude mousehTERT启动子调控TK基因靶向性杀伤鼻咽癌移植瘤的实验研究
14.Construction of Recombinant Lentiviral Expression Vector and Packaging of the Lentiviral Vectors by Means of Electroporation重组hTERT启动子启动CD基因慢病毒载体构建及电穿孔法包装慢病毒颗粒
15.The results suggest that TRAIL may be a promising target for gene therapy of NPC.在肿瘤研究中,TRAIL结合hTERT启动子可为肿瘤基因治疗的靶向性提供实验依据。
16.Experimental Studies on Therapy for Gastric Cancer with Replicative Adenovirus Driven by hTERT Promoter and Carrying Mouse Endostatin Gene;hTERT启动子调控的增殖型腺病毒携带内皮抑素基因治疗胃癌的实验研究
17.Construction and expression of lentivirus vector containing eGFP drived by hTERT promoterhTERT基因启动子驱动eGFP的慢病毒载体构建及表达的初步研究
18.Study of Tumor Cell Apoptosis in HSV/TK Suicide System Induced by hTERT Core Promoter;hTERT核心启动子在HSV/TK自杀基因系统中诱导肿瘤细胞凋亡的研究
相关短句/例句
the promotor of h-HTERTh-HTERT启动子
3)telomerase catalytic subunit core promoterhTERT核心启动子
1.Vascular endothelial growth factor enhancer and telomerase catalytic subunit core promoter purpose fragments were amplified by genome from human liver cancer cell.以人肝癌细胞基因组DNA为模板,应用PCR方法扩增VEGF增强子和hTERT核心启动子序列,该序列与NCBI数据库参考序列相比,同源性达到99%以上。
4)hTERT promotorhTERT基因启动子
1.Respecting that natural hTERT promotor is not enough excellent in transcription activity and specificity, we design our research to obtain a high specificity and transcription activity hTERT promotor that expressed in telomerase.然而天然hTERT启动子在转录活性与特异性方面不够理想,为获取一种在端粒酶表达阳性细胞中具有高特异性和较强转录活性的hTERT基因启动子,我们以天然hTERT基因编码序列启始子ATG上游270bp碱基序列为基本框架结构,进行启动子重组设计,在其下游接入四个E-box(CACGTG)重复序列结构,用化学合成法将该序列进行人工合成并亚克隆入PUC57克隆载体中;构建表达增强型绿色荧光蛋白(enhanced green fluorescent protein,eGFP)报告基因的慢病毒载体,并用重组的hTERT基因启动子片段取代慢病毒载体eGFP上游的巨细胞病毒(cytomegalovirus,CMV)启动子;将新得到的重组慢病毒载体用脂质体介导法转入端粒酶阳性的人胚肾上皮肿瘤细胞(293FT)、肝癌细胞(HepGⅡ。
5)hTERT gene core promoterhTERT基因核心启动子
1.Construction of TRAIL gene eukaryotic expression vector modulated by hTERT gene core promoter and its effect on apoptosis of ovarian cancer cells;hTERT基因核心启动子调控的TRAIL基因表达载体的构建及对卵巢癌细胞凋亡的影响
6)tissue-specific promoter Amy-2肿瘤靶向性hTERT启动子
延伸阅读
启动子分子式：CAS号：性质：(一)在DNA分子上，可被RNA聚合酶识别并与之结合的一段序列，位于结构基因的上游，在那里使转录起动。强的启动子可转录出更多的nRNA。因而在重组工作菌中常使用强制的启动子，以期取得更多的表达量。（二）一种致癌因素，在癌变过程的第二阶段或多阶段机制中造成第二阶段（促进阶段）；一种辅助致癌物。
