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【主权项】
1. 大豆紫色酸性磷酸酶GmPAP36，其特征在于，其氨基酸序列如SEQIDN0:l所示，或该 序列经替换、缺失或添加一个或几个氨基酸形成的具有同等功能的氨基酸序列。2. 权利要求1所述大豆紫色酸性磷酸酶GmPAP36的编码基因。3. 如权利要求2所述的基因，其特征在于，其为大豆紫色酸性磷酸酶GmPAP36基因，该基 因的cDNA序列如SEQ ID N0:2所示。4. 含有权利要求2或3所述基因的载体，其出发载体包括pCamE、ρΖΥΙΟ 1。5. 含有权利要求2或3所述基因或权利要求4所述载体的宿主细胞或工程菌。6. 权利要求2或3所述基因在提高转基因植物对环境中磷元素利用率中的应用。7. 根据权利要求6所述的应用，其特征在于，所述磷元素以有机态磷形式存在，优选地， 所述有机态磷为植酸磷。8. 根据权利要求6或7所述的应用，其特征在于，所述植物包括拟南芥、大豆。9. 权利要求2或3所述基因在植物品种改良中的应用，其中所述植物包括拟南芥、大豆。10. -种转基因植株的构建方法，其特征在于，采用农杆菌介导法或花粉管通道法，将 权利要求4所述的载体转入植物中，筛选转基因植株。
【专利摘要】本发明提供大豆紫色酸性磷酸酶GmPAP36、其编码基因及应用。本发明基于抑制性差减杂交技术构建低磷诱导大豆根系cDNA差减文库，从中选择低磷胁迫诱导表达的酸性磷酸酶候选EST，在此基础上，克隆酸性磷酸酶候选基因序列，采用实时定量PCR技术分析候选基因在植酸磷处理条件下的表达差异；构建基因原核表达载体，实现基因原核表达，同时构建基因真核表达载体，并转化拟南芥、大豆，获得转基因阳性植株，分析基因GmPAP36的生物学功能，为植物磷高效转基因育种提供功能基因。
【IPC分类】C12N15/55, C12N9/16, C12N15/82, A01H5/00
【公开号】CN105647884
【申请号】
【发明人】张彩英, 李喜焕, 孙星, 段鹏博, 孔佑宾, 杜汇, 李文龙
【申请人】河北农业大学
【公开日】2016年6月8日
【申请日】2016年2月2日